Unless your pH is ridiculously alkaline or acidic, the microbes will be fine. They’re soil microbes, not water microbes. Don’t leave them in water. Mix and use right away. The small volume used of Recharge solution is not going to change the pH of your media.

NEVER post pictures of just leaves. There is no context. We need to see the plant and where the issue is on the plant. Random damaged leaves say nothing.